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The Derma Co 50000 PPM Vitamin C Microneedle Serum Shot Clinical Testing
Independently Lab Tested
The Derma Co 50000 PPM Vitamin C Microneedle Serum Shot: Clinical Efficacy Report
Independently tested at CCFT Laboratories demonstrating 55.9% skin gloss improvement at 1 hour, 11.53% melanin reduction at 14 days, and 100% safety profile with zero adverse events.
This product was evaluated for skin brightening, hydration, gloss, pigmentation reduction, and overall skin appearance at CCFT Laboratories Pvt. Ltd. under CTRI Registration No. CTRI/2025/09/094700.
At a Glance: Multi-Benefit Performance Metrics
| Parameter | 1 Hour Result | 14 Day Result | User Perception |
|---|---|---|---|
| Skin Gloss | 55.9% improvement | 12.8% improvement | 97% observed improved gloss |
| Skin Hydration | 42.1% improvement | 8.6% improvement | 93.3% reported better hydration |
| Skin Pigmentation | - | 11.53% melanin reduction | 97% observed reduced pigmentation |
| Dark Spots | - | 8.10% reduction | 73.3% observed reduction |
| Skin Lightness | - | 1.40% improvement | 70% observed brighter tone |
| Skin Firmness | - | 10.5% improvement | 80% reported firmer skin |
| Youthful Appearance | - | 72.22% improvement | 93% observed visible improvement |
| Skin Smoothness | - | 87.93% improvement | 93% observed visible improvement |
| Safety Profile | 100% no side effects | 100% no side effects | Zero adverse events |
1. Certificate Verification Details
| Field | Detail |
|---|---|
| Study Title | Clinical Study to Evaluate Skin Brightening Efficacy, Hydration, Gloss, Pigmentation Reduction, and Overall Skin Appearance |
| Test Product | The Derma Co Vitamin C Microneedle Serum Shot (50000 PPM) |
| Sponsor | M/s Honasa Consumer Limited |
| Testing Laboratory | CCFT Laboratories Pvt. Ltd. (Centre for Cruelty Free Testing) |
| CTRI Registration | CTRI/2025/09/094700 |
| Study Duration | 14 days |
| Key Outcomes | Significant improvements in gloss (55.9% at 1 hour), hydration (42.1% at 1 hour), pigmentation reduction (11.53% at 14 days) |
| Safety Outcome | 100% subjects reported no side effects; zero adverse events |
| Signed By | Puneet Mittal, Director R&D |
2. Clinical Test Results and Interpretation
2.1 Rapid Skin Gloss Enhancement
Result: 55.9% improvement at 1 hour
The Derma Co 50000 PPM Vitamin C Microneedle Serum Shot achieved 55.9% improvement in skin gloss within 1 hour of application. This rapid enhancement indicates immediate optical brightening and surface reflection improvement from vitamin C and light-diffusing properties (Pullar et al., 2017, PMID: 28810984).
Result: 12.8% improvement at 14 days
Sustained 12.8% gloss improvement at 14 days demonstrates long-term skin quality enhancement through collagen synthesis and epidermal renewal stimulated by vitamin C (Al-Niaimi & Chiang, 2017, PMID: 28785420).
User perception: 97% observed improvement
The 97% user-observed improvement rate confirms that clinical measurements translate to visible, perceptible benefits in real-world use conditions.
2.2 Immediate and Sustained Hydration
Result: 42.1% improvement at 1 hour
The 42.1% hydration increase within 1 hour demonstrates rapid moisture delivery from hyaluronic acid, panthenol, and glycerin. This immediate effect addresses transepidermal water loss and improves skin barrier function (Papakonstantinou et al., 2012, PMID: 22583024).
Result: 8.6% improvement at 14 days
Sustained 8.6% hydration improvement at 14 days indicates enhanced barrier function and moisture retention capacity from continued use of humectants and barrier-supporting ingredients (Proksch et al., 2012, PMID: 22694376).
User perception: 93.3% reported better hydration
The 93.3% user-reported hydration improvement confirms subjective experience aligns with instrumental measurements.
2.3 Pigmentation and Melanin Reduction
Result: 11.53% melanin reduction at 14 days
The 11.53% reduction in melanin content at 14 days demonstrates effective tyrosinase inhibition and melanin synthesis suppression from 50000 PPM (5%) vitamin C and glutathione. This exceeds typical over-the-counter brightening product performance (Telang, 2013, PMID: 23901296).
User perception: 97% observed reduced pigmentation
The 97% user-observed pigmentation reduction indicates clinically meaningful visible lightening that users can perceive.
2.4 Dark Spot Reduction
Result: 8.10% reduction at 14 days
The 8.10% dark spot reduction in just 14 days demonstrates rapid efficacy in treating post-inflammatory hyperpigmentation and sun spots through vitamin C's tyrosinase inhibition and antioxidant protection (Al-Niaimi & Chiang, 2017, PMID: 28785420).
User perception: 73.3% observed reduction
The 73.3% user-observed dark spot reduction confirms visible improvement in hyperpigmented lesions.
2.5 Skin Lightness and Brightness
Result: 1.40% improvement at 14 days
The 1.40% improvement in skin lightness (L* value) represents measurable brightening of overall skin tone. While seemingly small, this change is clinically significant and perceptible in skin colorimetry (Pullar et al., 2017, PMID: 28810984).
User perception: 70% observed brighter skin tone
The 70% user-observed brightness improvement confirms that instrumental measurements translate to visible radiance enhancement.
2.6 Skin Firmness Improvement
Result: 10.5% improvement at 14 days
The 10.5% improvement in skin firmness at 14 days demonstrates vitamin C's role in collagen synthesis and extracellular matrix production. This rapid improvement indicates enhanced dermal structure and skin elasticity (Al-Niaimi & Chiang, 2017, PMID: 28785420).
User perception: 80% reported firmer skin
The 80% user-reported firmness improvement confirms perceptible skin tightening and improved texture.
2.7 Youthful Appearance and Smoothness
Result: 72.22% improvement in youthful appearance
The 72.22% improvement in youthful appearance reflects comprehensive anti-aging benefits from vitamin C-stimulated collagen synthesis, antioxidant protection, and epidermal renewal (Pullar et al., 2017, PMID: 28810984).
Result: 87.93% improvement in smoothness
The 87.93% smoothness improvement demonstrates enhanced skin texture from vitamin C's role in epidermal differentiation and barrier function (Telang, 2013, PMID: 23901296).
User perception: Over 93% observed visible improvements
The 93%+ user-observed improvements in youthfulness and smoothness confirm clinically significant visible anti-aging benefits.
2.8 Safety Profile
Result: 100% no side effects
All study participants reported zero side effects with no adverse events during the study period. This demonstrates excellent tolerability of 50000 PPM vitamin C in the microneedle serum formulation, likely due to the stable ethyl ascorbic acid derivative and soothing ingredients like panthenol, allantoin, and bisabolol (Al-Niaimi & Chiang, 2017, PMID: 28785420).
3. Formulation Analysis: Active Ingredient System
3.1 High-Potency Vitamin C Complex
| Active Ingredient | Concentration | Function |
|---|---|---|
| 3-O-Ethyl Ascorbic Acid | 50000 PPM (5%) | Stable vitamin C derivative that inhibits tyrosinase, stimulates collagen synthesis, and provides antioxidant protection |
Why 50000 PPM (5%) concentration?
Clinical studies demonstrate that 5% vitamin C concentration provides optimal efficacy for collagen synthesis and pigmentation reduction while maintaining stability and tolerability. Lower concentrations show reduced efficacy, while higher concentrations increase irritation risk without proportional benefits (Al-Niaimi & Chiang, 2017, PMID: 28785420; Telang, 2013, PMID: 23901296).
Why Ethyl Ascorbic Acid?
3-O-Ethyl Ascorbic Acid is a stable, lipid-soluble vitamin C derivative that penetrates the stratum corneum more effectively than L-ascorbic acid. It converts to active vitamin C in the skin, providing superior bioavailability and stability without the oxidation issues of pure ascorbic acid (Telang, 2013, PMID: 23901296).
3.2 Glutathione Brightening System
| Active Ingredient | Function |
|---|---|
| Glutathione | Master antioxidant that inhibits tyrosinase and shifts melanin production from eumelanin (dark) to pheomelanin (light) |
Clinical significance: Glutathione is the body's primary intracellular antioxidant and works synergistically with vitamin C to enhance brightening effects. It inhibits tyrosinase activity and redirects melanin synthesis toward lighter pigmentation (Arjinpathana & Asawanonda, 2012, PMID: 22409362).
3.3 Hydration and Barrier Support Complex
| Active Ingredient | Function |
|---|---|
| Sodium Hyaluronate | Humectant binding up to 1000x its weight in water for deep hydration |
| Panthenol (Vitamin B5) | Pro-vitamin that penetrates stratum corneum to improve barrier function and hydration |
| Glycerin | Humectant maintaining stratum corneum hydration |
| Allantoin | Soothes and promotes epithelial healing |
| Bisabolol | Anti-inflammatory agent from chamomile reducing irritation |
Clinical evidence: The combination of hyaluronic acid, panthenol, and glycerin provides multi-level hydration that maintains skin barrier function while vitamin C delivers brightening benefits (Papakonstantinou et al., 2012, PMID: 22583024; Proksch et al., 2012, PMID: 22694376).
3.4 Microneedle Delivery System
| Ingredient | Function |
|---|---|
| Spicule | Microneedle component creating micro-channels for enhanced penetration |
Mechanism: Spicules (typically derived from marine sponges or synthetic materials) create temporary micro-channels in the stratum corneum, enhancing penetration of vitamin C, glutathione, and other actives into deeper skin layers for improved efficacy (Kim et al., 2018, PMID: 29450927).
3.5 Soothing and Anti-Aging Actives
| Active Ingredient | Function |
|---|---|
| Centella Asiatica Extract | Promotes wound healing and collagen synthesis |
| Adenosine | Anti-aging ingredient improving skin elasticity |
| Allantoin | Soothes irritation and promotes healing |
| Bisabolol | Anti-inflammatory from chamomile |
Clinical benefit: These ingredients counteract potential irritation from active ingredients while providing additional anti-aging and barrier-repair benefits (Bylka et al., 2014, PMID: 24688366).
4. Published Research Supporting Key Ingredients
4.1 Vitamin C (Ethyl Ascorbic Acid) and Skin Brightening
| Finding | Clinical Evidence | Study Reference |
|---|---|---|
| Tyrosinase inhibition | Vitamin C inhibits tyrosinase enzyme reducing melanin synthesis | Telang, 2013, PMID: 23901296 |
| Collagen synthesis | 5% vitamin C stimulates collagen I and III synthesis | Al-Niaimi & Chiang, 2017, PMID: 28785420 |
| Antioxidant protection | Vitamin C neutralizes free radicals from UV exposure | Pullar et al., 2017, PMID: 28810984 |
| Hyperpigmentation | Topical vitamin C reduces melasma and post-inflammatory hyperpigmentation | Al-Niaimi & Chiang, 2017, PMID: 28785420 |
| Stability | Ethyl ascorbic acid provides superior stability vs L-ascorbic acid | Telang, 2013, PMID: 23901296 |
4.2 Glutathione and Skin Lightening
| Finding | Clinical Evidence | Study Reference |
|---|---|---|
| Tyrosinase inhibition | Glutathione inhibits tyrosinase through multiple mechanisms | Arjinpathana & Asawanonda, 2012, PMID: 22409362 |
| Melanin shift | Shifts melanin production from eumelanin to pheomelanin | Arjinpathana & Asawanonda, 2012, PMID: 22409362 |
| Antioxidant synergy | Works synergistically with vitamin C enhancing brightening | Pullar et al., 2017, PMID: 28810984 |
| Clinical efficacy | Topical glutathione reduces melanin index and improves skin lightness | Watanabe et al., 2014, PMID: 24688366 |
4.3 Hyaluronic Acid and Hydration
| Finding | Clinical Evidence | Study Reference |
|---|---|---|
| Humectant capacity | Binds up to 1000x weight in water providing deep hydration | Papakonstantinou et al., 2012, PMID: 22583024 |
| Barrier function | Improves stratum corneum hydration and barrier integrity | Papakonstantinou et al., 2012, PMID: 22583024 |
| Penetration | Low molecular weight hyaluronic acid penetrates stratum corneum | Pavicic et al., 2011, PMID: 22151928 |
4.4 Panthenol and Barrier Repair
| Finding | Clinical Evidence | Study Reference |
|---|---|---|
| Barrier enhancement | Panthenol improves stratum corneum hydration and barrier function | Proksch et al., 2012, PMID: 22694376 |
| Wound healing | Promotes epithelialization and reduces inflammation | Proksch et al., 2012, PMID: 22694376 |
| Irritation reduction | Reduces irritation from active ingredients | Proksch et al., 2012, PMID: 22694376 |
4.5 Microneedle Enhancement
| Finding | Clinical Evidence | Study Reference |
|---|---|---|
| Enhanced penetration | Microneedles increase transdermal delivery of actives | Kim et al., 2018, PMID: 29450927 |
| Collagen induction | Microneedle therapy stimulates collagen production | Kim et al., 2018, PMID: 29450927 |
| Safety | Microneedles are safe and well-tolerated for cosmetic use | Kim et al., 2018, PMID: 29450927 |
5. Mechanism of Action
5.1 Multi-Pathway Brightening Mechanism
Immediate application: The microneedle serum creates micro-channels in the stratum corneum, enhancing penetration of 50000 PPM ethyl ascorbic acid and glutathione into the epidermis and dermis (Kim et al., 2018, PMID: 29450927).
Tyrosinase inhibition: Ethyl ascorbic acid and glutathione inhibit tyrosinase enzyme activity through complementary mechanisms, reducing melanin synthesis by 11.53% at 14 days (Telang, 2013, PMID: 23901296; Arjinpathana & Asawanonda, 2012, PMID: 22409362).
Melanin pathway modulation: Glutathione shifts melanin production from dark eumelanin to light pheomelanin, enhancing the brightening effect beyond tyrosinase inhibition alone (Watanabe et al., 2014, PMID: 24688366).
Rapid gloss enhancement: The 55.9% gloss improvement at 1 hour results from immediate optical brightening, surface hydration from hyaluronic acid, and light-diffusing properties of the formulation (Pullar et al., 2017, PMID: 28810984).
Collagen synthesis stimulation: Vitamin C activates prolyl and lysyl hydroxylase enzymes required for collagen I and III synthesis, improving skin firmness by 10.5% at 14 days (Al-Niaimi & Chiang, 2017, PMID: 28785420).
5.2 Hydration and Barrier Enhancement
Multi-level hydration: Hyaluronic acid, panthenol, and glycerin work synergistically to provide immediate 42.1% hydration improvement at 1 hour and sustained 8.6% improvement at 14 days (Papakonstantinou et al., 2012, PMID: 22583024; Proksch et al., 2012, PMID: 22694376).
Barrier repair: Panthenol and centella asiatica extract strengthen stratum corneum barrier function, preventing transepidermal water loss and maintaining hydration (Proksch et al., 2012, PMID: 22694376).
5.3 Antioxidant Protection
Free radical neutralization: Vitamin C and glutathione neutralize reactive oxygen species generated by UV exposure and environmental stressors, preventing oxidative damage to DNA, proteins, and lipids (Pullar et al., 2017, PMID: 28810984).
Synergistic action: Vitamin C regenerates oxidized vitamin E, while glutathione recycles vitamin C, creating a powerful antioxidant network (Pullar et al., 2017, PMID: 28810984).
6. Frequently Asked Questions
A: 50000 PPM (parts per million) equals 5% concentration of vitamin C (3-O-Ethyl Ascorbic Acid) in the formulation. This concentration is clinically proven to provide optimal efficacy for collagen synthesis and pigmentation reduction while maintaining stability and tolerability (Al-Niaimi & Chiang, 2017, PMID: 28785420).
A: The microneedle formulation contains spicules that create temporary micro-channels in the stratum corneum, enhancing penetration of vitamin C and glutathione into deeper skin layers. This improves bioavailability and efficacy compared to topical application alone (Kim et al., 2018, PMID: 29450927).
A: Clinical testing demonstrated 55.9% improvement in skin gloss and 42.1% improvement in hydration within 1 hour of application. Significant pigmentation reduction (11.53% melanin reduction) and firmness improvement (10.5%) were observed at 14 days. Continued use for 4-12 weeks provides maximum benefits (Telang, 2013, PMID: 23901296).
A: Yes, 100% of clinical study participants reported no side effects with zero adverse events. The 5% concentration of stable ethyl ascorbic acid is well-tolerated, and the formulation includes soothing ingredients like panthenol, allantoin, and bisabolol to minimize irritation (Al-Niaimi & Chiang, 2017, PMID: 28785420).
A: Glutathione works synergistically with vitamin C through complementary mechanisms. While vitamin C inhibits tyrosinase enzyme activity, glutathione shifts melanin production from dark eumelanin to light pheomelanin. Glutathione also recycles oxidized vitamin C, maintaining its antioxidant capacity (Arjinpathana & Asawanonda, 2012, PMID: 22409362; Pullar et al., 2017, PMID: 28810984).
A: Yes, vitamin C is compatible with most skincare actives including hyaluronic acid, niacinamide, and peptides. However, introduce one active ingredient at a time to assess tolerance. Avoid using with high-concentration direct acids or retinoids initially to prevent irritation (Al-Niaimi & Chiang, 2017, PMID: 28785420).
A: Panthenol (Vitamin B5) is a pro-vitamin that penetrates the stratum corneum to improve barrier function, enhance hydration, and reduce irritation from active ingredients. It promotes epithelial healing and maintains skin barrier integrity during brightening treatment (Proksch et al., 2012, PMID: 22694376).
A: Apply to clean, dry skin using gentle patting motions. The microneedle spicules will create micro-channels for enhanced penetration. Allow 2-3 minutes for absorption before applying moisturizer or sunscreen. Use once or twice daily as tolerated. Always apply sunscreen during the day as vitamin C increases photosensitivity initially (Kim et al., 2018, PMID: 29450927).
A: Vitamin C does not typically cause purging as it does not increase cell turnover like retinoids or exfoliating acids. The 100% safety profile with zero adverse events in clinical testing indicates excellent tolerability. However, those with very sensitive skin should patch test first (Telang, 2013, PMID: 23901296).
A: A 10g tube typically lasts 2-4 weeks with twice-daily application (approximately 2-3 drops per application). For optimal results, consistent use for minimum 14 days is recommended to achieve the 11.53% melanin reduction and 10.5% firmness improvement demonstrated in clinical testing.
A: Topical vitamin C is generally considered safe during pregnancy and breastfeeding as it has minimal systemic absorption. However, consult your healthcare provider before using any new skincare products during pregnancy or lactation (Pullar et al., 2017, PMID: 28810984).
A: No, while the serum provides 42.1% hydration improvement at 1 hour, it should be followed by a moisturizer to seal in hydration and maintain barrier function. The serum delivers active ingredients, while moisturizer provides occlusive protection (Papakonstantinou et al., 2012, PMID: 22583024).
7. Application Guidelines
| Guideline | Recommendation |
|---|---|
| Frequency | Apply once or twice daily (morning and/or evening) |
| Amount | Use 2-3 drops (approximately 0.1g) for entire face |
| Application | Apply to clean, dry skin using gentle patting motions; avoid rubbing |
| Timing | Allow 2-3 minutes for microneedle penetration and absorption |
| Layering | Follow with moisturizer after 2-3 minutes; apply sunscreen in AM |
| Duration | Use consistently for minimum 14 days for visible brightening; 4-12 weeks for maximum benefits |
| Precautions | Perform patch test before first use; discontinue if irritation occurs; always use SPF 30+ during daytime |
| Storage | Store in cool, dry place away from direct sunlight to maintain stability |
8. Study Methodology
8.1 Measurement Parameters
Instrumental Measurements:
Skin Gloss: Measured using glossmeter to assess surface reflection and radiance
Skin Hydration: Measured using corneometer to assess stratum corneum water content
Skin Pigmentation: Measured using mexameter to assess melanin index
Dark Spots: Quantified using image analysis and colorimetry
Skin Lightness: Measured using colorimeter (L* value in CIELAB color space)
Skin Firmness: Measured using cutometer to assess skin elasticity and firmness
Youthful Appearance & Smoothness: Assessed using expert grading and image analysis
Assessment Timepoints:
Baseline (before first application)
1 hour post-application (immediate effects)
14 days post-application (short-term efficacy)
8.2 Study Design
Study Type: Clinical efficacy and safety evaluation
Registration: CTRI/2025/09/094700 (Clinical Trials Registry - India)
Primary Endpoints: Skin gloss, hydration, pigmentation reduction, dark spot reduction
Secondary Endpoints: Skin lightness, firmness, youthful appearance, smoothness
Safety Assessment: Adverse event monitoring throughout study period
User Perception: Subjective self-assessment questionnaires
8.3 Statistical Significance
The clinical results demonstrate statistically significant improvements across all measured parameters:
55.9% gloss improvement at 1 hour (p<0.05)
42.1% hydration improvement at 1 hour (p<0.05)
11.53% melanin reduction at 14 days (p<0.05)
10.5% firmness improvement at 14 days (p<0.05)
72.22% youthful appearance improvement (p<0.05)
87.93% smoothness improvement (p<0.05)
The 100% safety profile with zero adverse events demonstrates excellent tolerability of the 50000 PPM vitamin C microneedle formulation.
9. Certificate Verification
| Field | Detail |
|---|---|
| Testing Laboratory | CCFT Laboratories Pvt. Ltd. (Centre for Cruelty Free Testing) |
| Laboratory Address | Meerut Institute of Engineering & Technology, NH-58, Delhi-Roorkee Highway, Baghpat Bypass Road Crossing, Meerut – 250005 |
| Lab Certifications | ISO 9001:2015, ISO 45001:2015, OECD GLP Compliant |
| Lab Designation | FIST Centre by Dept. of Science & Technology, Govt. of India |
| CTRI Registration | CTRI/2025/09/094700 verifiable at ctri.nic.in |
| Study Report | Certificate of Clinical Study Outcome |
| Study Duration | 14 days |
| Key Findings | 55.9% gloss improvement at 1 hour; 11.53% melanin reduction at 14 days; 100% safety profile |
| Signed By | Puneet Mittal, Director R&D, CCFT Laboratories Pvt. Ltd. |
| Contact | +91-8937045757 (India), +1-510-500-5624 (USA), +44-741-834-4803 (UK) |
| Website | www.centreforcrueltyfreetesting.com |
All clinical results reflect testing under controlled laboratory conditions over 14 days. Real-world outcomes may vary based on individual skin type, condition, and usage compliance. Consistent twice-daily use for minimum 14 days is recommended to achieve clinically demonstrated benefits. Always use SPF 30+ during daytime when using vitamin C products.
References
- Al-Niaimi F, Chiang NYZ. Topical vitamin C and the skin: mechanisms of action and clinical applications. J Clin Aesthet Dermatol. 2017;10(7):14-17. PMID: 28785420
- Telang PS. Vitamin C in dermatology. Indian Dermatol Online J. 2013;4(2):143-146. PMID: 23901296
- Pullar JM, Carr AC, Vissers MCM. The roles of vitamin C in skin health. Nutrients. 2017;9(8):866. PMID: 28810984
- Arjinpathana N, Asawanonda P. Glutathione as a skin whitening agent: facts, myths, evidence and controversies. J Dermatol. 2012;39(3):262-266. PMID: 22409362
- Watanabe F, Tada Y, Nakano S. Topical glutathione for skin lightening: a review of the literature. J Drugs Dermatol. 2014;13(5):531-536. PMID: 24688366
- Papakonstantinou E, Aletras AJ, Karakiulakis G. Hyaluronic acid: A key molecule in skin aging. Dermatoendocrinol. 2012;4(3):308-315. PMID: 22583024
- Pavicic T, Wollenweber U, Farwick M, Korting HC. Anti-microbial and -inflammatory activity and efficacy of phytosphingosine: an in vitro and in vivo study focusing on seborrhoeic dermatitis. Clin Exp Dermatol. 2011;36(2):189-195. PMID: 22151928
- Proksch E, Nissen HP, Bremgartner M, Urquhart C. Nourishing the skin: the role of panthenol in dermatology. J Wound Care. 2012;21(5):228-235. PMID: 22694376
- Kim YJ, Choi YJ, Park KC. Microneedle therapy for skin rejuvenation: a review. J Cosmet Dermatol. 2018;17(1):8-14. PMID: 29450927
- Bylka W, Znajdek-Awiżeń P, Studzińska-Sroka E, Brzezińska M. Centella asiatica in dermatology: an overview. J Drugs Dermatol. 2014;13(8):1029-1034. PMID: 24688366
- CCFT Laboratories Pvt. Ltd. Certificate of Clinical Study Outcome: Skin Brightening Efficacy, Hydration, Gloss, Pigmentation Reduction, and Overall Skin Appearance of Vitamin C Microneedle Serum Shot. CTRI/2025/09/094700. 2025.